Inhibition by Pyridoxal-Phosphate of Glyceraldehyde-3-Phosphate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
Acetyl phosphate formation catalyzed by glyceraldehyde-3-phosphate dehydrogenase.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the oxidative phosphorylation of n-glyceraldehyde-3-phosphate to 1,3-diphosphoglyceric acid (1, 2). The enzyme also catalyzes the oxidation of n-glyceraldehyde, although the product of this reaction has not been characterized (1,2). In the present study it is shown that acetaldehyde (3), propionaldehyde, and butyraldehyde also act as substrates...
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The catalytic interaction of glyceraldehyde-3-phosphate dehydrogenase with glyceraldehyde 3-phosphate has been examined by transient-state kinetic methods. The results confirm previous reports that the apparent Km for oxidative phosphorylation of glyceraldehyde 3-phosphate decreases at least 50-fold when the substrate is generated in a coupled reaction system through the action of aldolase on f...
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifaceted protein that is involved in numerous processes including glycolysis, translational silencing, transcriptional regulation of specific genes, and acting as a nitric oxide sensor. The precise mechanism on how GAPDH is targeted to these different roles is unclear but believed to involve specific posttranslational modification to the...
متن کاملTransfer reactions of acetyl phosphate catalyzed by glyceraldehyde-3-phosphate dehydrogenase.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the oxidative phosphorylation of acetaldehyde (1) and a number of other aliphatic aldehydes (2, 3) and also has a transacetylating activity (4). In the present paper the reactions of acetyl phosphate which characterize the transacetylating activity of the enzyme are presented. These reactions include (a) the phosphate exchange between acetyl ph...
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Ewa Seweryn3*, A rtur Pędyczakb and Teresa Banaś3 a Wroclaw Medical University, Department of Medical Biochemistry, Chalubiriskiego 10, 50-368 Wroclaw. Poland. Fax: (+48) 0713285415. E-mail: [email protected] b University of Wroclaw, Department of Chemistry, Wroclaw, Poland * Author for correspondence and reprint requests Z. Naturforsch. 55c, 657-660 (2000); received January 10/April 11, 20...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1969
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1969.tb00531.x